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Article

Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer.

Nature communications - 9 Aug 2017

Gao Ruli, Kim Charissa, Sei Emi, Foukakis Theodoros, Crosetto Nicola, Chan Leong-Keat, Srinivasan Maithreyan, Zhang Hong, Meric-Bernstam Funda, Navin Nicholas

Abstract excerpt

Single cell RNA sequencing has emerged as a powerful tool for resolving transcriptional diversity in tumors, but is limited by throughput, cost and the ability to process archival frozen tissue samples. Here we develop a high-throughput 3' single-nucleus RNA sequencing approach that combines nanogrid technology, automated imaging, and cell selection to sequence up to ~1800 single nuclei in parallel. We compare...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28794488
DOI: 10.1038/s41467-017-00244-w
PMCID: PMC5550415

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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