Article
Allele specific locked nucleic acid quantitative PCR (ASLNAqPCR): an accurate and cost-effective assay to diagnose and quantify KRAS and BRAF mutation.
PloS one - 1 Jan 2012
Morandi Luca, de Biase Dario, Visani Michela, Cesari Valentina, De Maglio Giovanna, Pizzolitto Stefano, Pession Annalisa, Tallini Giovanni
Abstract excerpt
The use of tyrosine kinase inhibitors (TKIs) requires the testing for hot spot mutations of the molecular effectors downstream the membrane-bound tyrosine kinases since their wild type status is expected for response to TKI therapy. We report a novel assay that we have called Allele Specific Locked Nucleic Acid quantitative PCR (ASLNAqPCR). The assay uses LNA-modified allele specific primers and LNA-modified...
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