Article
Evolved Escherichia coli strains for amplified, functional expression of membrane proteins.
Journal of molecular biology - 9 Jan 2014
Gul Nadia, Linares Daniel M, Ho Franz Y, Poolman Bert
Abstract excerpt
The major barrier to the physical characterization and structure determination of membrane proteins is low protein yield and/or low functionality in recombinant expression. The enteric bacterium Escherichia coli is the most widely employed organism for producing recombinant proteins. Beside several advantages of this expression host, one major drawback is that the protein of interest does not always adopt its...
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