Article
Improved Dynamic Range of a Rhamnose-Inducible Promoter for Gene Expression in Burkholderia spp.
Applied and environmental microbiology - 26 Aug 2021
Hogan Andrew M, Jeffers Kevin R, Palacios Armando, Cardona Silvia T
Abstract excerpt
A diverse genetic toolkit is critical for understanding bacterial physiology and genotype-phenotype relationships. Inducible promoter systems are an integral part of this toolkit. In Burkholderia and related species, the l-rhamnose-inducible promoter is among the first choices due to its tight control and the lack of viable alternatives. To improve upon its maximum activity and dynamic range, we explored the...
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