Short, auto-inducible promoters for well-controlled protein expression in Escherichia coli.

Abstract excerpt

Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to trigger the protein expression during early exponential growth phase. This practice has many...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Escherichia coli
• Share to Gene Expression Regulation, Bacterial