Article
Assessment of the quality of DNA from various formalin-fixed paraffin-embedded (FFPE) tissues and the use of this DNA for next-generation sequencing (NGS) with no artifactual mutation.
PloS one - 1 Jan 2017
Einaga Naoki, Yoshida Akio, Noda Hiroko, Suemitsu Masaaki, Nakayama Yuki, Sakurada Akihisa, Kawaji Yoshiko, Yamaguchi Hiromi, Sasaki Yasushi, Tokino Takashi, Esumi Mariko
Abstract excerpt
Formalin-fixed, paraffin-embedded (FFPE) tissues used for pathological diagnosis are valuable for studying cancer genomics. In particular, laser-capture microdissection of target cells determined by histopathology combined with FFPE tissue section immunohistochemistry (IHC) enables precise analysis by next-generation sequencing (NGS) of the genetic events occurring in cancer. The result is a new strategy for a...
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