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Article

Primerize-2D: automated primer design for RNA multidimensional chemical mapping.

Bioinformatics (Oxford, England) - 1 May 2017

Tian Siqi, Das Rhiju

Abstract excerpt

Summary: Rapid RNA synthesis of comprehensive single mutant libraries and targeted multiple mutant libraries is enabling new multidimensional chemical approaches to solve RNA structures. PCR assembly of DNA templates and in vitro transcription allow synthesis and purification of hundreds of RNA mutants in a cost-effective manner, with sharing of primers across constructs allowing significant reductions in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 28453672
DOI: 10.1093/bioinformatics/btw814
PMCID: PMC5859995

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