Article
Quinolone resistance-associated amino acid substitutions affect enzymatic activity of Mycobacterium leprae DNA gyrase.
Bioscience, biotechnology, and biochemistry - 1 Jul 2017
Yamaguchi Tomoyuki, Yokoyama Kazumasa, Nakajima Chie, Suzuki Yasuhiko
Abstract excerpt
Quinolones are important antimicrobials for treatment of leprosy, a chronic infectious disease caused by Mycobacterium leprae. Although it is well known that mutations in DNA gyrase are responsible for quinolone resistance, the effect of those mutations on the enzymatic activity is yet to be studied in depth. Hence, we conducted in vitro assays to observe supercoiling reactions of wild type and mutated M. leprae...
Topics
- Amino Acid Substitution
- Anti-Bacterial Agents
- Cloning, Molecular
- DNA Gyrase
- DNA, Bacterial
- DNA, Circular
- DNA, Superhelical
- Drug Resistance, Bacterial
- Enzyme Assays
- Escherichia coli