Article
Amide hydrogens reveal a temperature-dependent structural transition that enhances site-II Ca2+-binding affinity in a C-domain mutant of cardiac troponin C.
Scientific reports - 6 Apr 2017
Veltri Tiago, de Oliveira Guilherme A P, Bienkiewicz Ewa A, Palhano Fernando L, Marques Mayra de A, Moraes Adolfo H, Silva Jerson L, Sorenson Martha M, Pinto Jose R
Abstract excerpt
The hypertrophic cardiomyopathy-associated mutant D145E, in cardiac troponin C (cTnC) C-domain, causes generalised instability at multiple sites in the isolated protein. As a result, structure and function of the mutant are more susceptible to higher temperatures. Above 25 °C there are large, progressive increases in N-domain Ca2+-binding affinity for D145E but only small changes for the wild-type protein....
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