Article
Using Morpholinos to Examine Gene Function During Fin Regeneration.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Thummel Ryan, Kathryn Iovine M
Abstract excerpt
In the zebrafish regenerating fin, specific gene-targeting morpholinos have been widely utilized to assess gene function. Unlike in embryos, injection of standard morpholinos in the adult regenerating fin is not sufficient for cellular uptake. Rather, morpholinos are first injected extracellularly into the blastemal compartment, followed by electroporation for cellular uptake. Knockdown phenotypes are evaluated...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
