Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology.

Abstract excerpt

Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Genetic Techniques
• Share to Genome
• Share to Morpholinos