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Article

Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development.

Journal of visualized experiments : JoVE - 9 Aug 2022

Zakaria Zain Zaki, Eisa-Beygi Shahram, Benslimane Fatiha M, Ramchandran Ramani, Yalcin Huseyin C

Abstract excerpt

The morpholino oligomer-based knockdown system has been used to identify the function of various gene products through loss or reduced expression. Morpholinos (MOs) have the advantage in biological stability over DNA oligos because they are not susceptible to enzymatic degradation. For optimal effectiveness, MOs are injected into 1-4 cell stage embryos. The temporal efficacy of knockdown is variable, but MOs are...

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Share to Embryo, Nonmammalian
Share to Gene Knockdown Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Oligonucleotides, Antisense

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Identifiers

PMID: 36036621
DOI: 10.3791/63324
PMCID: PMC10388372

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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