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Article

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach.

Journal of visualized experiments : JoVE - 12 Mar 2017

Dhoondia Zuzer, Tarockoff Ricci, Alhusini Nadra, Medler Scott, Agarwal Neha, Ansari Athar

Abstract excerpt

This manuscript describes a protocol for detecting transcription termination defect in vivo. The strand-specific TRO protocol using BrUTP described here is a powerful experimental approach for analyzing the transcription termination defect under physiological conditions. Like the traditional TRO assay, it relies on the presence of a transcriptionally active polymerase beyond the 3' end of the gene as an indicator...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28362387
DOI: 10.3791/55446
PMCID: PMC5408991

Genes

TRO

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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