Article
Termination-altering mutations in the second-largest subunit of yeast RNA polymerase III.
Molecular and cellular biology - 1 Mar 1995
Shaaban S A, Krupp B M, Hall B D
Abstract excerpt
In order to identify catalytically important amino acid changes within the second-largest subunit of yeast RNA polymerase III, we mutagenized selected regions of its gene (RET1) and devised in vivo assays for both increased and decreased transcription termination by this enzyme. Using as the repo...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Coatomer Protein
- Macromolecular Substances
- Membrane Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Phenotype
- Plasmids
- RNA Polymerase III
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Terminator Regions, Genetic
- Transcription, Genetic