Article
Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3).
Scientific reports - 24 Mar 2017
Baumgarten Thomas, Schlegel Susan, Wagner Samuel, Löw Mirjam, Eriksson Jonas, Bonde Ida, Herrgård Markus J, Heipieper Hermann J, Nørholm Morten H H, Slotboom Dirk Jan, de Gier Jan-Willem
Abstract excerpt
Membrane protein production is usually toxic to E. coli. However, using genetic screens strains can be isolated in which the toxicity of membrane protein production is reduced, thereby improving production yields. Best known examples are the C41(DE3) and C43(DE3) strains, which are both derived from the T7 RNA polymerase (P)-based BL21(DE3) protein production strain. In C41(DE3) and C43(DE3) mutations lowering...
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