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Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity.

Communications biology - 12 Aug 2021

Heyde Sophia A H, Nørholm Morten H H

Abstract excerpt

Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance to toxic genes. However, previous efforts to evolve BL21, such as the Walker strains C41 and C43, resulted only in...

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PMID: 34385596
DOI: 10.1038/s42003-021-02493-4
PMCID: PMC8361080

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