Article
Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli.
FEMS microbiology letters - 1 Jun 1998
Prilipov A, Phale P S, Van Gelder P, Rosenbusch J P, Koebnik R
Abstract excerpt
Combination of an origin repair mutagenesis system with a new mutS host strain increased the efficiency of mutagenesis from 46% to 75% mutant clones. Overexpression with the T7 expression system afforded large quantities of proteins from mutant strains. A series of E. coli BE host strains devoid of major outer membrane proteins was constructed, facilitating the purification of mutant porins to homogeneity. This...
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