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Article

Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction.

Scientific reports - 4 Nov 2015

Kwon Soon-Kyeong, Kim Seong Keun, Lee Dae-Hee, Kim Jihyun F

Abstract excerpt

Achieving sufficient yields of proteins in their functional form represents the first bottleneck in contemporary bioscience and biotechnology. To accomplish successful overexpression of membrane proteins in a workhorse organism such as E. coli, defined and rational optimization strategies based on an understanding of the genetic background of the toxicity-escape mechanism are desirable. To this end, we sequenced...

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Identifiers

PMID: 26531007
DOI: 10.1038/srep16076
PMCID: PMC4632034

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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