Article

Construction of Probiotic Escherichia coli Nissle 1917 as a Next- Generation Expression Platform and Its Application in Therapeutic Protein Production

2025-09-19

Abstract excerpt

<title>Abstract</title> Biologics produced in <italic>Escherichia coli</italic> BL21(DE3) require rigorous removal of lipopolysaccharide (LPS), also termed endotoxin, as its presence can elicit severe immune responses and life threatening complications in humans. <italic>Escherichia coli</italic> Nissle 1917 (EcN) is a clinically approved probiotic with unique biosafety characteristics due to its LPS-def...

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Literature Corpus work: b55845cc-b140-5a9e-a7eb-5e6bc86ff923
DOI: 10.21203/rs.3.rs-7512809/v1

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