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A novel suicide shuttle plasmid for Streptococcus suis serotype 2 and Streptococcus equi ssp. zooepidemicus gene mutation.

Scientific reports - 3 Jun 2016

Liu Rui, Zhang Ping, Su Yiqi, Lin Huixing, Zhang Hui, Yu Lei, Ma Zhe, Fan Hongjie

Abstract excerpt

The mariner-based Himar1 system has been utilized for creating mutant libraries of many Gram-positive bacteria. Streptococcus suis serotype 2 (SS2) and Streptococcus equi ssp. zooepidemicus (SEZ) are primary pathogens of swine that threaten the swine industry in China. To provide a forward-genetics technology for finding virulent phenotype-related genes in these two pathogens, we constructed a novel...

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PMID: 27256117
DOI: 10.1038/srep27133
PMCID: PMC4891806

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