Construction and characterization of a Streptomyces rimosus recA mutant: the RecA-deficient strain remains viable.

doi:10.1007/s004380000284

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Article

Construction and characterization of a Streptomyces rimosus recA mutant: the RecA-deficient strain remains viable.

Molecular & general genetics : MGG - 1 Oct 2000

Mikoc A, Ahel I, Gamulin V

Abstract excerpt

Although previously reported attempts to construct recA null mutants in Streptomyces spp. have been unsuccessful, we have used the suicide plasmid pErmdeltaRecA to inactivate the recA gene in Streptomyces rimosus by gene disruption. pErmdeltaRecA carries the erythromycin resistance gene ermE and a 451-bp fragment of the S. rimosus recA gene (encoding amino acids 2-151). An erythromycin-resistant clone with single...

Topics

Blotting, Southern
Codon, Initiator
Dose-Response Relationship, Radiation
Drug Resistance, Microbial
Electroporation
Erythromycin
Gene Deletion
Models, Genetic
Mutagenesis, Site-Directed
Mutation
Plasmids
Promoter Regions, Genetic
Rec A Recombinases
Streptomyces
Ultraviolet Rays

Source

Open in PubMed

Identifiers

PMID: 11085261
DOI: 10.1007/s004380000284

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