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Article

Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans.

Proceedings of the National Academy of Sciences of the United States of America - 14 Mar 2017

Nayak Dipti D, Metcalf William W

Abstract excerpt

Although Cas9-mediated genome editing has proven to be a powerful genetic tool in eukaryotes, its application in Bacteria has been limited because of inefficient targeting or repair; and its application to Archaea has yet to be reported. Here we describe the development of a Cas9-mediated genome-editing tool that allows facile genetic manipulation of the slow-growing methanogenic archaeon Methanosarcina...

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Identifiers

PMID: 28265068
DOI: 10.1073/pnas.1618596114
PMCID: PMC5358397

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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