Article
High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea.
Journal of microbiological methods - 1 Dec 2016
Walter Mary E, Ortiz Alicia, Sondgeroth Casey, Sindt Nathan M, Duszenko Nikolas, Catlett Jennie L, Zhou You, Valloppilly Shah, Anderson Christopher, Fernando Samodha, Buan Nicole R
Abstract excerpt
Bacterial and archaeal genomes can contain 30% or more hypothetical genes with no predicted function. Phylogenetically deep-branching microbes, such as methane-producing archaea (methanogens), contain up to 50% genes with unknown function. In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we have developed high-throughput...
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