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Article

Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea.

Applied and environmental microbiology - 1 Mar 2004

Pritchett Matthew A, Zhang Jun Kai, Metcalf William W

Abstract excerpt

A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed. Mutants with lesions in the hpt gene, encoding hypoxanthine phosphoribosyltransferase, were shown to be >35-fold more resistant to the toxic base analog 8-aza-2,6-diaminopurine (8ADP) than was the wild type. Reintroduction of the hpt gene into a Delta hpt host restored 8ADP...

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Identifiers

PMID: 15006762
DOI: 10.1128/AEM.70.3.1425-1433.2004
PMCID: PMC368415

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