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Article

Isolation of acetate auxotrophs of the methane-producing archaeon Methanococcus maripaludis by random insertional mutagenesis.

Genetics - 1 Aug 1999

Kim W, Whitman W B

Abstract excerpt

To learn more about autotrophic growth of methanococci, we isolated nine conditional mutants of Methanococcus maripaludis after transformation of the wild type with a random library in pMEB.2, a suicide plasmid bearing the puromycin-resistance cassette pac. These mutants grew poorly in mineral medium and required acetate or complex organic supplements such as yeast extract for normal growth. One mutant, JJ104,...

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Source

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Identifiers

PMID: 10430573
DOI: 10.1093/genetics/152.4.1429
PMCID: PMC1460683

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Source receipt: pubmed, retrieved 7 Sept 2026.

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