Article
Dissection of the angle of single fluorophore attached to the nucleotide in corkscrewing microtubules.
Biochemical and biophysical research communications - 8 Apr 2017
Fujimura Shoko, Ito Yuko, Ikeguchi Mitsunori, Adachi Kengo, Yajima Junichiro, Nishizaka Takayuki
Abstract excerpt
Direct dissection of the angles of single fluorophores under an optical microscope has been a challenging approach to study the dynamics of proteins in an aqueous solution. For angle quantifications of single substrates, however, there was only one report (Nishizaka et al., 2014) because of difficulties of construction of experimental systems with active proteins working at the single-molecule level. We here show...
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