Article
Performance comparison of next-generation sequencing platforms for determining HIV-1 coreceptor use.
Scientific reports - 10 Feb 2017
Raymond Stéphanie, Nicot Florence, Jeanne Nicolas, Delfour Olivier, Carcenac Romain, Lefebvre Caroline, Cazabat Michelle, Sauné Karine, Delobel Pierre, Izopet Jacques
Abstract excerpt
The coreceptor used by HIV-1 must be determined before a CCR5 antagonist, part of the arsenal of antiretroviral drugs, is prescribed because viruses that enter cells using the CXCR4 coreceptor are responsible for treatment failure. HIV-1 tropism is also correlated with disease progression and so must be determined for virological studies. Tropism can be determined by next-generation sequencing (NGS), but not all...
Topics
- Base Sequence
- HIV Infections
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Mutation
- Phenotype
- Receptors, CXCR4
- Receptors, HIV
- Viral Tropism