Article
Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.
Nature communications - 10 Jan 2017
Chauvier Adrien, Picard-Jean Frédéric, Berger-Dancause Jean-Christophe, Bastet Laurène, Naghdi Mohammad Reza, Dubé Audrey, Turcotte Pierre, Perreault Jonathan, Lafontaine Daniel A
Abstract excerpt
On the basis of nascent transcript sequencing, it has been postulated but never demonstrated that transcriptional pausing at translation start sites is important for gene regulation. Here we show that the Escherichia coli thiamin pyrophosphate (TPP) thiC riboswitch contains a regulatory pause site in the translation initiation region that acts as a checkpoint for thiC expression. By biochemically probing nascent...
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