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Article

Impact of Mutations on the Higher Order Structure and Activity of a Recombinant Uricase.

Journal of pharmaceutical sciences - 1 Apr 2017

Gruia Flaviu, Parupudi Arun, Baca Manuel, Ward Chris, Nyborg Andrew, Remmele Richard L, Bee Jared S

Abstract excerpt

This study explores the structural and functional changes associated with a low-temperature thermal transition of 2 engineered bacterial uricase mutants. Uricase has a noncovalent homotetrameric structure, with 4 active sites located at the interface of subunits. Using differential scanning calorimetry, a low-temperature transition was identified at 42°C for mutant A and at 33°C for mutant B. This transition was...

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Identifiers

PMID: 28063825
DOI: 10.1016/j.xphs.2016.12.028

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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