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Article

Rational design approach to improve the solubility of the β-sandwich domain 1 of a thermophilic protein.

Journal of bioscience and bioengineering - 1 Oct 2024

Ononugbo Chukwuebuka M, Shimura Yusaku, Yamano-Adachi Noriko, Omasa Takeshi, Koga Yuichi

Abstract excerpt

The β-sandwich domain 1 (SD1) of islandisin is a stable thermophilic protein with surface loops that can be redesigned for specific target binding, architecturally comparable to the variable domain of immunoglobulin (IgG). SD1's propensity to aggregate due to incorrect folding and subsequent accumulation in Escherichia coli inclusion bodies limits its use in biotechnological applications. We rationally designed...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39074993
DOI: 10.1016/j.jbiosc.2024.06.009

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