Article
Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy.
Scientific reports - 24 Nov 2016
Tochio Naoya, Umehara Kohei, Uewaki Jun-Ichi, Flechsig Holger, Kondo Masaharu, Dewa Takehisa, Sakuma Tetsushi, Yamamoto Takashi, Saitoh Takashi, Togashi Yuichi, Tate Shin-Ichi
Abstract excerpt
Transcription activator-like effector (TALE) nuclease (TALEN) is widely used as a tool in genome editing. The DNA binding part of TALEN consists of a tandem array of TAL-repeats that form a right-handed superhelix. Each TAL-repeat recognises a specific base by the repeat variable diresidue (RVD) at positions 12 and 13. TALEN comprising the TAL-repeats with periodic mutations to residues at positions 4 and 32...
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