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Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Salamon Daniel

Abstract excerpt

Characterization of viral DNA methylation patterns is essential to understand its function in viral pathogenesis. Bisulfite modification, followed by polymerase chain reaction (PCR) and sequencing is the most effective method for the high resolution methylation mapping of viral genomes. Since the bisulfite modification and PCR steps are the most critical ones, an optimized protocol for these two steps is...

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Share to DNA Methylation
Share to Epigenesis, Genetic
Share to Epigenomics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genome, Viral

Share to Herpesvirus 4, Human

Share to Promoter Regions, Genetic

Share to Sequence Analysis, DNA

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Identifiers

PMID: 27873278
DOI: 10.1007/978-1-4939-6655-4_15

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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