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Article

Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku.

Nature communications - 10 Nov 2016

Baym Michael, Shaket Lev, Anzai Isao A, Adesina Oluwakemi, Barstow Buz

Abstract excerpt

Whole-genome knockout collections are invaluable for connecting gene sequence to function, yet traditionally, their construction has required an extraordinary technical effort. Here we report a method for the construction and purification of a curated whole-genome collection of single-gene transposon disruption mutants termed Knockout Sudoku. Using simple combinatorial pooling, a highly oversampled collection of...

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Identifiers

PMID: 27830751
DOI: 10.1038/ncomms13270
PMCID: PMC5109470

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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