Article
Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq.
Molecular microbiology - 1 Oct 2012
Brutinel Evan D, Gralnick Jeffrey A
Abstract excerpt
The availability of increasingly inexpensive sequencing combined with an ever-expanding molecular biology toolbox has transported classical bacterial genetics into the 21st century. Whole genome genetic fitness analysis using transposon mutagenesis combined with next-generation high-throughput sequencing (Tn-seq) promises to revolutionize systems level analysis of microbial metabolism. Tn-seq measures the...
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