Article
Protein charge determination and implications for interactions in cell extracts.
Protein science : a publication of the Protein Society - 1 Feb 2017
Kyne Ciara, Jordon Kiara, Filoti Dana I, Laue Thomas M, Crowley Peter B
Abstract excerpt
Decades of dilute-solution studies have revealed the influence of charged residues on protein stability, solubility and stickiness. Similar characterizations are now required in physiological solutions to understand the effect of charge on protein behavior under native conditions. Toward this end, we used free boundary and native gel electrophoresis to explore the charge of cytochrome c in buffer and in...
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