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Article

Effects of reversing the protein positive charge in the proximity of the flavin N(1) locus of choline oxidase.

Biochemistry - 14 Mar 2006

Ghanem Mahmoud, Gadda Giovanni

Abstract excerpt

A protein positive charge near the flavin N(1) locus is a distinguishing feature of most flavoprotein oxidases, with mechanistic implications for the modulation of flavin reactivity. A recent study showed that in the active site of choline oxidase the protein positive charge is provided by His(466). Here, we have reversed the charge by substitution with aspartate (CHO-H466D) and, for the first time, characterized...

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Identifiers

PMID: 16519539
DOI: 10.1021/bi052514m

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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