Article
Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Nogi Terukazu, Mihara Emiko, Yasui Norihisa, Takagi Junichi
Abstract excerpt
Plexins are type I membrane proteins that function as receptors for semaphorins. All of the known plexins contain a large globular domain, termed the sema domain, in the N-terminal extracellular region, which interacts with semaphorins during signal transduction. Here, we describe procedures for protein production and purification that we utilized in the crystallographic study of the mouse Plexin A2 (mPlxnA2)...
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