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Article

Mechanistic insights into the manganese-dependent phosphodiesterase activity of yeast Dbr1 with bis-p-nitrophenylphosphate and branched RNA substrates.

RNA (New York, N.Y.) - 1 Dec 2016

Schwer Beate, Khalid Fahad, Shuman Stewart

Abstract excerpt

Saccharomyces cerevisiae Dbr1 is a manganese-dependent RNA debranching enzyme that cleaves the 2'-5' phosphodiester bond of the lariat introns formed during pre-mRNA splicing. Dbr1 is a member of the binuclear metallophosphoesterase enzyme superfamily. We showed previously via alanine scanning that RNA debranching in vivo and in vitro depends on conserved active site residues His13, Asp40, Asn85, His86, His179,...

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Identifiers

PMID: 27765821
DOI: 10.1261/rna.058552.116
PMCID: PMC5113202

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Source receipt: pubmed, retrieved 7 Sept 2026.

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