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Article

Metal requirements and phosphodiesterase activity of tRNase Z enzymes.

Biochemistry - 25 Dec 2007

Späth Bettina, Settele Florian, Schilling Oliver, D'Angelo Igor, Vogel Andreas, Feldmann Ingo, Meyer-Klaucke Wolfram, Marchfelder Anita

Abstract excerpt

The endonuclease tRNase Z from A. thaliana (AthTRZ1) was originally isolated for its tRNA 3' processing activity. Here we show that AthTRZ1 also hydrolyzes the phosphodiester bond in bis(p-nitrophenyl) phosphate (bpNPP) with a kcat of 7.4 s-1 and a KM of 8.5 mM. We analyzed 22 variants of AthTRZ1 with respect to their ability to hydrolyze bpNPP. This mutational mapping identified fourteen variants that lost the...

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PMID: 18052196
DOI: 10.1021/bi7010459

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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