Article
Scanning the mitochondrial genome for mutations by cycling temperature capillary electrophoresis.
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis - 1 Jan 2018
Arstad Christian, Refinetti Paulo, Warren David, Giercksky Karl-Erik, Ekstrøm Per Olaf
Abstract excerpt
To bypass possible nuclear contamination and to exclusively amplify DNA from the mitochondrion, a set of 23 primers was selected. On the mitochondrial DNA selection fragments, a second set of fragments was used to amplify and identify mutant fractions with a detection limit of 1% . This mutation scanning method analyzed 76% of the mitochondrial genome and was used to examine 94 tumours from different tissues of...
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