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Article

Sensitive detection of mitochondrial DNA variants for analysis of mitochondrial DNA-enriched extracts from frozen tumor tissue.

Scientific reports - 2 Feb 2018

Weerts M J A, Timmermans E C, Vossen R H A M, van Strijp D, Van den Hout-van Vroonhoven M C G N, van IJcken W F J, van der Zaag P J, Anvar S Y, Sleijfer S, Martens J W M

Abstract excerpt

Large variation exists in mitochondrial DNA (mtDNA) not only between but also within individuals. Also in human cancer, tumor-specific mtDNA variation exists. In this work, we describe the comparison of four methods to extract mtDNA as pure as possible from frozen tumor tissue. Also, three state-of-the-art methods for sensitive detection of mtDNA variants were evaluated. The main aim was to develop a procedure to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29396409
DOI: 10.1038/s41598-018-20623-7
PMCID: PMC5797170

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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