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Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA

30 Sept 2016

Abstract excerpt

Influenza viruses have a segmented viral RNA (vRNA) genome, which is replicated by the viral RNA-dependent RNA polymerase (RNAP). Replication initiates on the vRNA 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the synthesis of new vRNA. RNAP structures show the 3' terminus of the vRNA template in a pre-initiation state, bound on the surface of the RNAP rather than...

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PMID: 27694620
DOI: 10.1093/nar/gkw884

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