Article
A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker
28 Jan 2017
Abstract excerpt
Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d -psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis . In this study, the d -alanine racemase-encoding gene dal was deleted...
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