Construction of a Food Grade Recombinant <i>Bacillus subtilis</i> Based on Replicative Plasmids with an Auxotrophic Marker for Biotransformation of <scp>d</scp>-Fructose to <scp>d</scp>-Allulose

Abstract excerpt

A food grade recombinant Bacillus subtilis that produces d-psicose 3-epimerase (DPEase; EC 5.1.3.30) was constructed by transforming a replicative multicopy plasmid with a d-alanine racemase gene marker into B. subtilis 1A751 with the d-alanine racemase gene knocked out. The DPEase was expressed in B. subtilis without antibiotic resistance genes and without adding antibiotics during fermentation. Whole cells of...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Diet, Metabolism, and Disease