Article
T cell repertoire following kidney transplantation revealed by high-throughput sequencing.
Transplant immunology - 1 Nov 2016
Lai Liusheng, Wang Lei, Chen Huaizhou, Zhang Jiaxing, Yan Qiang, Ou Minglin, Lin Hua, Hou Xianliang, Chen Sisi, Dai Yong, Sui Weiguo
Abstract excerpt
Delayed T cell recovery and restricted T cell receptor (TCR) diversity after kidney transplantation are associated with increased risks of infection and malignancy. Technical challenges limit the faithful measurement of TCR diversity after kidney transplantation. In this study, we used a combination of multiplex-PCR, Illumina sequencing and IMGT/HighV-QUEST to directly assess millions of TCRs per individual...
Topics
- Adult
- Clonal Deletion
- Cohort Studies
- Complementarity Determining Regions
- Female
- Genetic Variation
- Graft Rejection
- High-Throughput Nucleotide Sequencing
- Humans
- Kidney Transplantation
- Male
- Middle Aged
- Monitoring, Immunologic
- Receptors, Antigen, T-Cell