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Article

Attempts to develop an enzyme converting DHIV to KIV.

Protein engineering, design & selection : PEDS - 31 Dec 2019

Oki Kenji, Lee Frederick S, Mayo Stephen L

Abstract excerpt

Dihydroxy-acid dehydratase (DHAD) catalyzes the dehydration of R-2,3-dihydroxyisovalerate (DHIV) to 2-ketoisovalerate (KIV) using an Fe-S cluster as a cofactor, which is sensitive to oxidation and expensive to synthesize. In contrast, sugar acid dehydratases catalyze the same chemical reactions using a magnesium ion. Here, we attempted to substitute the high-cost DHAD with a cost-efficient engineered sugar acid...

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PMID: 31872250
DOI: 10.1093/protein/gzz042

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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