Article
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Science (New York, N.Y.) - 16 Sept 2016
Nishida Keiji, Arazoe Takayuki, Yachie Nozomu, Banno Satomi, Kakimoto Mika, Tabata Mayura, Mochizuki Masao, Miyabe Aya, Araki Michihiro, Hara Kiyotaka Y, Shimatani Zenpei, Kondo Akihiko
Abstract excerpt
The generation of genetic variation (somatic hypermutation) is an essential process for the adaptive immune system in vertebrates. We demonstrate the targeted single-nucleotide substitution of DNA using hybrid vertebrate and bacterial immune systems components. Nuclease-deficient type II CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated) and the activation-induced cytidine...
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