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Article

Next-Generation Genotyping by Digital PCR to Detect and Quantify the BRAF V600E Mutation in Melanoma Biopsies.

The Journal of molecular diagnostics : JMD - 1 Jul 2015

Lamy Pierre-Jean, Castan Florence, Lozano Nicolas, Montélion Cécile, Audran Patricia, Bibeau Frédéric, Roques Sylvie, Montels Frédéric, Laberenne Anne-Claire

Abstract excerpt

The detection of the BRAF V600E mutation in melanoma samples is used to select patients who should respond to BRAF inhibitors. Different techniques are routinely used to determine BRAF status in clinical samples. However, low tumor cellularity and tumor heterogeneity can affect the sensitivity of somatic mutation detection. Digital PCR (dPCR) is a next-generation genotyping method that clonally amplifies nucleic...

Topics

Biopsy
DNA Mutational Analysis
DNA, Neoplasm
High-Throughput Nucleotide Sequencing
Humans
Melanoma
Mutation
Polymerase Chain Reaction
Proto-Oncogene Proteins B-raf
Skin Neoplasms

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Melanoma, Cutaneous Malignant

Source

Identifiers

PMID: 25952101
DOI: 10.1016/j.jmoldx.2015.02.004

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25952101-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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