Development of a 3D Tissue Culture–Based High-Content Screening Platform That Uses Phenotypic Profiling to Discriminate Selective Inhibitors of Receptor Tyrosine Kinases

Abstract excerpt

3D tissue cultures provide a more physiologically relevant context for the screening of compounds, compared with 2D cell cultures. Cells cultured in 3D hydrogels also show complex phenotypes, increasing the scope for phenotypic profiling. Here we describe a high-content screening platform that uses invasive human prostate cancer cells cultured in 3D in standard 384-well assay plates to study the activity of...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to 3D Printing in Biomedical Research
• Share to Cancer Cells and Metastasis
• Share to Viral Infectious Diseases and Gene Expression in Insects