Article
[Location of amino acid substitutions in human hemoglobin. Mass spectrometric rapid analysis of tryptic peptides].
Molekuliarnaia biologiia - 1 Jan 2000
Molchanova T P, Mirgorodskaia O A, Abaturov L V, Podtelezhnikov A V, Tokarev Iu N
Abstract excerpt
Point mutations alpha 58 His----Tyr (Hb M Boston), beta 6 Glu--lys (Hb C) and beta 26 Glu----Lys (Hb E) have been identified in abnormal hemoglobins by means of tryptic hydrolysis of their alpha- and beta-chains followed by mass-spectrometry coupled with direct extraction of ions from solution. The abnormal hemoglobin Hb M Boston alpha 58 (E7) His----Tyr has been for the first time found in the blood of a patient...
Topics
- Amino Acids
- Chromatography, High Pressure Liquid
- Electrophoresis, Cellulose Acetate
- Hemoglobins, Abnormal
- Humans
- Hydrolysis
- Kinetics
- Mass Spectrometry
- Mutation
- Peptide Fragments
- Peptide Mapping